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KMID : 0903519950380030224
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 3 p.224 ~ p.231
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Translational Enhancement by the 5´ Leader of Tobacco Mosaic Virus and Soybean Glycinin Gene in Transgenic Tobacco Plants
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Abstract
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To increase the expression of a foreign protein in transgenic plant, the benefits of 5´-untranslated leader sequences of tobacco mosaic virus (TMV) RNA or soybean glycinin gene, Gy2, fused to a protein coding sequence were exploited. pGA643-derived plasmid contains 35S promoter of cauliflower mosaic virus, protein coding sequence of maize 10 kDa zero (10kZ) and Gy2 terminator. The leader from Gy2 or TMV RNA was inserted between the promoter and the coding sequence in each construct. The recombinant DNAs were introduced into tobacco plants by Agrobacterium mediated leaf disc transformation method. Although the transgene without the leader had more transcripts than the others, mRNAs containing the leader were translated more efficiently. It might be due to difference in the length of 5´-untranslated sequence and context surrounding the AUG colon, but could be sequence specific rather. These results suggest that the leader sequences of Gy2 and TMV play important roles as an enhancer in translational control of foreign gene in transgenic tobacco plant.
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